gapdh (cat Search Results


gapdh (fwd 50-cat gag aag tat gac aac agc ct-30, rev 50-agt cct tcc acg ata cca aag t-30)  (biomers.net gmbh)
90
biomers.net gmbh gapdh (fwd 50-cat gag aag tat gac aac agc ct-30, rev 50-agt cct tcc acg ata cca aag t-30)
Gapdh (Fwd 50 Cat Gag Aag Tat Gac Aac Agc Ct 30, Rev 50 Agt Cct Tcc Acg Ata Cca Aag T 30), supplied by biomers.net gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh (fwd 50-cat gag aag tat gac aac agc ct-30, rev 50-agt cct tcc acg ata cca aag t-30)/product/biomers.net gmbh
Average 90 stars, based on 1 article reviews
gapdh (fwd 50-cat gag aag tat gac aac agc ct-30, rev 50-agt cct tcc acg ata cca aag t-30) - by Bioz Stars, 2026-04
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rabbit anti-rat primary antibodies targeted against gapdh (cat. no. 10494-1-ap)  (Wuhan Sanying Biotechnology)
90
Wuhan Sanying Biotechnology rabbit anti-rat primary antibodies targeted against gapdh (cat. no. 10494-1-ap)
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Rabbit Anti Rat Primary Antibodies Targeted Against Gapdh (Cat. No. 10494 1 Ap), supplied by Wuhan Sanying Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-rat primary antibodies targeted against gapdh (cat. no. 10494-1-ap)/product/Wuhan Sanying Biotechnology
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rabbit anti-rat primary antibodies targeted against gapdh (cat. no. 10494-1-ap) - by Bioz Stars, 2026-04
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antibody gapdh ac054  (ABclonal Biotechnology)
90
ABclonal Biotechnology antibody gapdh ac054
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Antibody Gapdh Ac054, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody gapdh ac054/product/ABclonal Biotechnology
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antibody gapdh ac054 - by Bioz Stars, 2026-04
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gapdh srp00849 antibody  (Tianjin Saier Biotechnology)
90
Tianjin Saier Biotechnology gapdh srp00849 antibody
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Gapdh Srp00849 Antibody, supplied by Tianjin Saier Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh srp00849 antibody/product/Tianjin Saier Biotechnology
Average 90 stars, based on 1 article reviews
gapdh srp00849 antibody - by Bioz Stars, 2026-04
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gapdh for ggt gtg aac cat gag aag tat ga  (BianoScience gmbh)
90
BianoScience gmbh gapdh for ggt gtg aac cat gag aag tat ga
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Gapdh For Ggt Gtg Aac Cat Gag Aag Tat Ga, supplied by BianoScience gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh for ggt gtg aac cat gag aag tat ga/product/BianoScience gmbh
Average 90 stars, based on 1 article reviews
gapdh for ggt gtg aac cat gag aag tat ga - by Bioz Stars, 2026-04
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the primers for gapdh were as follows: reverse primer, 5′-aca​cca​tgt​att​ccg​ggt​caa​t-3′; forward primer, 5′-tgt​ggg​cat​caa​tgg​att​tgg-3′  (Servicebio Inc)
90
Servicebio Inc the primers for gapdh were as follows: reverse primer, 5′-aca​cca​tgt​att​ccg​ggt​caa​t-3′; forward primer, 5′-tgt​ggg​cat​caa​tgg​att​tgg-3′
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
The Primers For Gapdh Were As Follows: Reverse Primer, 5′ Aca​Cca​Tgt​Att​Ccg​Ggt​Caa​T 3′; Forward Primer, 5′ Tgt​Ggg​Cat​Caa​Tgg​Att​Tgg 3′, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/the primers for gapdh were as follows: reverse primer, 5′-aca​cca​tgt​att​ccg​ggt​caa​t-3′; forward primer, 5′-tgt​ggg​cat​caa​tgg​att​tgg-3′/product/Servicebio Inc
Average 90 stars, based on 1 article reviews
the primers for gapdh were as follows: reverse primer, 5′-aca​cca​tgt​att​ccg​ggt​caa​t-3′; forward primer, 5′-tgt​ggg​cat​caa​tgg​att​tgg-3′ - by Bioz Stars, 2026-04
90/100 stars
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gapdh antibody cat. no. 21612  (Signalway Antibody)
90
Signalway Antibody gapdh antibody cat. no. 21612
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Gapdh Antibody Cat. No. 21612, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh antibody cat. no. 21612/product/Signalway Antibody
Average 90 stars, based on 1 article reviews
gapdh antibody cat. no. 21612 - by Bioz Stars, 2026-04
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mouse anti-gapdh cat. no. 5g4, clone 4g5  (HyTest)
90
HyTest mouse anti-gapdh cat. no. 5g4, clone 4g5
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Mouse Anti Gapdh Cat. No. 5g4, Clone 4g5, supplied by HyTest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-gapdh cat. no. 5g4, clone 4g5/product/HyTest
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mouse anti-gapdh cat. no. 5g4, clone 4g5 - by Bioz Stars, 2026-04
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gapdh hytest cat# 5g4; rrid: ab_2858176 antibody  (HyTest)
90
HyTest gapdh hytest cat# 5g4; rrid: ab_2858176 antibody
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Gapdh Hytest Cat# 5g4; Rrid: Ab 2858176 Antibody, supplied by HyTest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh hytest cat# 5g4; rrid: ab_2858176 antibody/product/HyTest
Average 90 stars, based on 1 article reviews
gapdh hytest cat# 5g4; rrid: ab_2858176 antibody - by Bioz Stars, 2026-04
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human gapdh standard probe set panomics cat. no. sa-10001-01  (Panomics Inc)
90
Panomics Inc human gapdh standard probe set panomics cat. no. sa-10001-01
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Human Gapdh Standard Probe Set Panomics Cat. No. Sa 10001 01, supplied by Panomics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human gapdh standard probe set panomics cat. no. sa-10001-01/product/Panomics Inc
Average 90 stars, based on 1 article reviews
human gapdh standard probe set panomics cat. no. sa-10001-01 - by Bioz Stars, 2026-04
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gapdh (cat #gb1100c2)  (Servicebio Inc)
90
Servicebio Inc gapdh (cat #gb1100c2)
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Gapdh (Cat #Gb1100c2), supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh (cat #gb1100c2)/product/Servicebio Inc
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gapdh (cat #gb1100c2) - by Bioz Stars, 2026-04
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gapdh (cat. no. hqp006940)  (Guangzhou Fulengen Co)
90
Guangzhou Fulengen Co gapdh (cat. no. hqp006940)
Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of <t>Wnt-1</t> and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.
Gapdh (Cat. No. Hqp006940), supplied by Guangzhou Fulengen Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh (cat. no. hqp006940)/product/Guangzhou Fulengen Co
Average 90 stars, based on 1 article reviews
gapdh (cat. no. hqp006940) - by Bioz Stars, 2026-04
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Image Search Results


Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of Wnt-1 and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on MNNG-stimulated tumor-related indexes in WB-F344 cells. (A) Expression of CK-19 protein in each treatment group was detected by flow cytometry. (B) Concentration of AFP protein in the supernatant of each treatment group was detected by performing ELISA. (C) Expression of Wnt-1 and β-catenin protein in each group was detected by performing an immunofluorescence assay (magnification, ×400). Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein; cy, cyanine.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Expressing, Flow Cytometry, Concentration Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Effect of KXRG-containing serum on the Wnt-1/β-catenin signaling pathway in MNNG-stimulated WB-F344 cells. mRNA expression levels of (A) Wnt-1, β-catenin, Cyclin D1, (B) C-myc, MMP-7, Axin2 and EpCAM were detected via reverse transcription-quantitative PCR. Protein expression levels of (C) Wnt-1, β-catenin, Cyclin D1, (D) C-myc, MMP-7, Axin2 and EpCAM were detected by performing western blotting. The data of the normal group are set to 1 and data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; MMP, matrix metalloproteinase; EpCAM, epithelial cell adhesion molecule.

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on the Wnt-1/β-catenin signaling pathway in MNNG-stimulated WB-F344 cells. mRNA expression levels of (A) Wnt-1, β-catenin, Cyclin D1, (B) C-myc, MMP-7, Axin2 and EpCAM were detected via reverse transcription-quantitative PCR. Protein expression levels of (C) Wnt-1, β-catenin, Cyclin D1, (D) C-myc, MMP-7, Axin2 and EpCAM were detected by performing western blotting. The data of the normal group are set to 1 and data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; MMP, matrix metalloproteinase; EpCAM, epithelial cell adhesion molecule.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Concentration Assay

Effect of KXRG-containing serum on the apoptotic rate and cell cycle in MNNG-stimulated WB-F344 cells following Wnt-1 overexpression. (A) Protein expression level of Wnt-1 was detected by performing western blotting to confirm Wnt-1 overexpression in WB-F344 cells. (B) Flow cytometry was performed to detect the apoptotic rate of each group. (C) The cell cycle of each group was detected by flow cytometry. Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model; ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; NC, negative control.

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on the apoptotic rate and cell cycle in MNNG-stimulated WB-F344 cells following Wnt-1 overexpression. (A) Protein expression level of Wnt-1 was detected by performing western blotting to confirm Wnt-1 overexpression in WB-F344 cells. (B) Flow cytometry was performed to detect the apoptotic rate of each group. (C) The cell cycle of each group was detected by flow cytometry. Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model; ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; NC, negative control.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Over Expression, Expressing, Western Blot, Flow Cytometry, Concentration Assay, Negative Control

Effect of KXRG-containing serum on CK-19 and AFP in MNNG-stimulated, Wnt-1-overexpression WB-F344 cells. CK-19 protein expression was (A) detected by flow cytometry and (B) quantified. (C) Concentration of AFP protein in the supernatant of each group. Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal. *P<0.01 vs. model. ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein.

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on CK-19 and AFP in MNNG-stimulated, Wnt-1-overexpression WB-F344 cells. CK-19 protein expression was (A) detected by flow cytometry and (B) quantified. (C) Concentration of AFP protein in the supernatant of each group. Data are presented as the mean ± SD (n=3). # P<0.05 vs. normal. *P<0.01 vs. model. ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; CK, cytokeratin; AFP, α-fetoprotein.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Over Expression, Expressing, Flow Cytometry, Concentration Assay

Effect of KXRG-containing serum on MNNG-stimulated WB-F344 cells following Wnt-1 overexpression. The expression of the Wnt-1 and β-catenin proteins in each group was detected by performing an immunofluorescence assay (magnification, ×400). KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; cy, cyanine.

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on MNNG-stimulated WB-F344 cells following Wnt-1 overexpression. The expression of the Wnt-1 and β-catenin proteins in each group was detected by performing an immunofluorescence assay (magnification, ×400). KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; cy, cyanine.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Over Expression, Expressing, Immunofluorescence, Concentration Assay

Effect of KXRG-containing serum on the Wnt-1/β-catenin signaling pathway in MNNG-stimulated, Wnt-1-overexpression WB-F344 cells. mRNA expression levels of (A) Wnt-1, β-catenin, Cyclin D1, (B) C-myc, MMP-7, Axin2 and EpCAM were detected by reverse transcription-quantitative PCR. Protein expression levels of (C) Wnt-1, β-catenin, Cyclin D1, (D) C-myc, MMP-7, Axin2, and EpCAM detected by western blotting. The data of the normal group are set to 1 and data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model; ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; MMP, matrix metalloproteinase; EpCAM, epithelial cell adhesion molecule.

Journal: Molecular Medicine Reports

Article Title: Kangxianruangan granule-containing serum mediated inhibition of hepatic oval cell differentiation into hepatocellular carcinoma cells via the Wnt-1/β-catenin signaling pathway

doi: 10.3892/mmr.2021.12571

Figure Lengend Snippet: Effect of KXRG-containing serum on the Wnt-1/β-catenin signaling pathway in MNNG-stimulated, Wnt-1-overexpression WB-F344 cells. mRNA expression levels of (A) Wnt-1, β-catenin, Cyclin D1, (B) C-myc, MMP-7, Axin2 and EpCAM were detected by reverse transcription-quantitative PCR. Protein expression levels of (C) Wnt-1, β-catenin, Cyclin D1, (D) C-myc, MMP-7, Axin2, and EpCAM detected by western blotting. The data of the normal group are set to 1 and data are presented as the mean ± SD (n=3). # P<0.05 vs. normal; *P<0.01 vs. model; ※P<0.01 vs. Wnt-1-up. KXRG, kangxianruangan granule; MNNG, N-methyl-N′-nitro-N-nitrosoguanidine; KXRG-H, high concentration of KXRG-containing serum; KXRG-M, middle concentration of KXRG-containing serum; KXRG-L, low concentration of KXRG-containing serum; MMP, matrix metalloproteinase; EpCAM, epithelial cell adhesion molecule.

Article Snippet: Rabbit anti-rat primary antibodies targeted against Wnt-1 (cat. no. 27935-1-AP), β-catenin (cat. no. 66379-1-Ig), Cyclin D1 (cat. no. 60186-1-Ig), C-myc (cat. no. 67447-1-Ig), matrix metalloproteinase-7 (MMP-7; cat. no. 10374-2-AP), Axin2 (cat. no. 20540-1-AP), epithelial cell adhesion molecule (EpCAM; cat. no. 21050-1-AP) and GAPDH (cat. no. 10494-1-AP), as well as HRP-labeled goat anti-rabbit secondary antibodies (cat. no. PR30009) were purchased from Wuhan Sanying Biotechnology.

Techniques: Over Expression, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Concentration Assay